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KMID : 0366220060410010008
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Korean Journal of Hematology
2006 Volume.41 No. 1 p.8 ~ p.15
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Selective Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells by K562 Cell Line and IL-2
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Cho Duck
Shin Shi-Won
Park Jung-Sun
Kang Hyun-Kyu
Kim Sang-Ki
Than-Nhan Nguyen Pham
Xiao-Wei Zhu
Shin Myung-Geun
Suh Soon-Pal
Ryang Dong-Wook
Nam Jong-Hee
Kim Young-Jin
Lee Je-Jung
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Abstract
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Background: Several attempts have been made to expand human NK cells from peripheral blood mononuclear cells (PBMCs). This study examined the selective expansion of NK cells using interleukin 2 (IL-2) plus the K562 cell line, the expression of the NK cell receptors, and the cytotoxic activity.
Methods: The PBMCs from seven healthy volunteers were cultured in a medium containing the IL-2 plus the K562 cell line for 14 days. The expression of the activating and inhibitory receptors on the resting NK cells and the 72 hr-expanded NK cells were analyzed. A flow cytometric cytotoxic assay was used to determined the killing activity of the non-expanded NK cells and the 7 day-expanded NK cells against the K562 target cells.
Results: The NK cells from PBMCs expanded 4.5-fold after 7 days, and contained 56.5% CD3-CD56+ cells. The IL-2 or IL-2 plus K562 increased the expression levels of CD158b (MFI, mean florescence intensity), CD158e1/e2 (MFI), and NKp44 (MFI), while it decreased the expression levels of NKp30 (%), CD16 (MFI), and 2B4 (MFI). The non-expanded NK cells lysed 9.0% and 27.6% of the K562 target cells in the 1：1 and 5：1 effector and target ratio, respectively, and the 7-day expanded NK cells lysed 36.9% and 57.2% of the K562 target cells, respectively.
Conclusion: The selective expansion of CD3-CD56+ NK cells occurred only during 7 days of culture. IL-2 or IL-2 plus the K562 cells altered the expression of various activating and inhibitory receptors of NK cells, and the cytotoxicity of the expanded NK cells was higher than in the non-expanded cells. (Korean J Hematol 2006;41:8-15.)
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KEYWORD
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Natural killer cells, K562, IL-2, Expansion
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